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A soluble Tie2 receptor transcript was delivered via an adenoviral vector to mice.
Such combinational therapy has been shown for alpha1-antitrypsin (AAT) deficiency, in which a hairpin RNA targeting mutated AAT transcript was delivered together with codon-optimized AAT gene [ 29].
Strikingly, the stimulated transcript was delivered equally well to both branches.
An efficient shRNA sequence specific to AIMP-1 transcripts was delivered to HEK 293 cells, which were afterwards transfected with the CRT-expressing vector (euCRT).
After Stroud's death, the transcripts were delivered to his lawyer, Richard English.
A spokesman for the MoD said the document was a "speaking note" rather than a transcript of Gould's speech, which was delivered in June last year, adding that it did not reflect government policy.
A transcript was released today.
Later, a transcript was filed publicly.
A transcript was released on Thursday.
Transcript will be delivered by email as a PDF attachment or WeTransfer, depending on file size.
Several non-viral approaches have been taken, including plasmid delivery in liposomes and other nanoparticles, but this results in very inefficient delivery of transcripts to T cells; transcripts that are delivered in this way are also non-integrative, limiting the longevity of their therapeutic value.
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