Exact(3)
This is a useful first approximation; further analysis could include miRNA data and other regulatory factors that would be expected to influence the rate of translation of a transcript to a protein.
This similarity implies repeatability of the CSH between the RNA and the microarray probes, despite low homology matches of a transcript to a spot, or where more than one transcript hybridizes to one spot.
The most widely applied algorithms including miRanda [ 13], Pictar [ 10] and TargetScan [ 14] use a combination of sequence complementarity of a transcript to a conserved region at the 5' end of the miRNA spanning position 2 to position 7 (known as the "seed" region), thermodynamic feasibility of hybridisation and evolutionary conservation [ 1].
Similar(54)
The process began with an analyst reading a transcript to get an overview of its contents.
As an additional criterion, for a transcript to be considered significantly up regulated, it was required that P d > 0.05.
We found almost all unmethylated CGIs (95%) associated with a transcript to be promoter-associated, and a large majority (85%) of the transcript-associated methylated CGIs were intragenic.
We have modelled the gene expression profiles of the TFs and their targets to estimate the likelihood of a transcript to be a target of JunD/AP-1 complexes.
Hybridisation accessibility profile of the first 120 nt in the coding region of the human EGFR mRNA was determined by hybridising a radiolabelled EGFR transcript to a scanning array of 2684 antisense sequences ranging from monomers to 27-mers.
Most of the steps required to mature a nascent transcript to a functional mRNA take place while the transcript is still attached to elongating polymerase.
In addition, since FAS undergoes multiple alternative splicing [ 40], it is impossible to assign a splice variant transcript to a protein species.
But past attempts to decipher keyboard sounds were only modestly successful, requiring a training session in which the computer matched a known transcript to an audio recording of each key being struck.
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