If a transcript existed of their 57-year-long marriage and you did a computer quick-find search of "Ducky," you'd find 1,794,326 matches.
The eagerness of many opponents, including Democrats on the Judiciary Committee, to obtain the speech has grown out of the uncertainty of whether a transcript existed.
Historians had never known that a transcript existed for the event held in the heat of World War II, when delegates from 44 allied nations fighting Hitler gathered in the mountains of New Hampshire to create the International Monetary Fund and the World Bank.
Primers 1F and 1R amplify a region within gene 1, while 2F and 2R amplify a region within gene 2. When a continuous transcript exists, 1F and 2R amplify across the intergenic region between genes 1 and 2. Therefore, the size of the end-point RT-PCR product will be the same using either cDNA or genomic DNA as templates.
Put another way, if such a putative transcript exists and induces the observed long-range correlation between gene expression signals by binding to multiple probe sets, it cannot be a typical protein-encoding transcript, irrespective of whether its affinity to the probe sets is high or low.
According to current annotation for the ACE gene (NCBI), a third ACE transcript exists.
For some lincRNAs, even just a few or a single transcript exist in a cell, determined by RNA-Seq data [ 10].
The results were consistent with findings obtained from quantitative phz transcript analysis by QRT-PCR, but were not consistent with the PCA quantity produced in wild type strain M18 or in the two mutants, suggesting a repression mechanism involved in the phzA1-G1 trandcript anotnot in the phzA2-G2 transcript exists at the post-transcriptional levels in strain M18.
(No transcript exists, which means future Hollywood screenwriters will have to compose their own).
After being told that no such transcript exists, the foreman said the panel did not need it.
