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RNA binding experiments were performed using guide concentrations that ranged between 0.08 and 0.64 µM and 0.32 µM of unlabelled target RNA spiked with a trace of labeled target in 20 µl reaction buffer (30 mM Tris pH 7.5, 150 mM KCl, 5 mM spermidine, 10 mM MgCl2, 0.1 mM dithiothreitol (DTT), and 0.1 mM EDTA pH 7.5) for 5 minutes at 30°C.
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Whereas substantial amounts of labeled TAG were formed in the presence of the phospholipid mixture used for the acyl-CoA independent assay (see Materials and methods), only traces of labeled TAG were formed when individual phospholipids (phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phophatidylserine) were used as acyl-donor.
Hardly any trace of label was found at depth of the CF and PM treatment.
Although this study traces the distribution of a population of labeled axons, the trajectories of individual axons were noted when they could be followed.
A minor signal labeled at m/ z 962.73 is indicative of a trace of peptide without fucosylation.
Not a trace of mud.
A trace of cushion.
Without a trace of a smile, Mlle.
There was a trace of woodworm damage.
A trace of mineral smoke rises.
They're poetic, a trace of a trace".
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