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Genome-wide association analysis, implemented in Merlin, was conducted on standardized scores using a total test of association which corrected for sample relatedness (Chen & Abecasis 2007).
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Each of the four subscales contains six test items for a total test length of 24 items.
The tests were conducted at temperatures above 850 °C and for a total test duration of 22 h.
This alternative to the standard method produces a total testing time of 3.58 hrs, including mechanical lysis (42 min) and detection (139 min).
The bottles are changed once per hour over a total testing time of 4 h.
A total of seven tests of significance were carried out using χ2 tests of significance, five site-specific comparisons and two branch-site comparisons were performed.
This led to a total of 341 tests of stability, with 270 and 71 tests for Cat 1 and Cat 2, respectively.
Ignoring the global tests and proceeding with haplotype-specific analyses for each of the six outcomes would have resulted in a total of 198 tests of association.
This resulted in a total of 102 tests, of which 14 were found to be significant at a p-value of <0.01 (Kendall Tau rank correlation test).
Therefore a total of 80 tests of primary stability were conducted, 40 each for the hemispherical and PSL designs, using four hemispherical and four PSL components.
The MCAT is a challenging exam with a total content test time of four hours and five minutes.
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