We obtained a total read of 70,544,933 from three experimental sets, each of which was replicated 3 times (Table 1).
In this study, two small RNA libraries were sequenced, and a total reads of 9,705,761 and 9,005,563 were generated from developing seeds and growing leaves, respectively.
The libraries were sequenced from one end of the molecules to a total read length of 100 nt.
This resulted in a total read length of 35,035,388 nucleotides and a total unique length of 10,420,310 nucleotides that were mapped to the assembly.
The average read length was 425 bp with a total read length of 355,789,178 bp.
For NA12760 CTRL, 14 × 2 haplotypes were inferred with a total read ratio of 104:97 (1.07:1; additional file 16).
Gene expression was represented as reads per million (RPM), which corresponds to (total reads of a given gene/total reads in the tissue) × 10.
Lowly supported transcripts, accounting for less than 1% of total reads of a gene (IsoPct), were discarded.
The total number of variants found for each library was subjected to an abundance filter which consisted of choosing variants that had a total number of reads 50% greater than the total reads of their reference miRNA previously reported.
The high throughput sequencing yielded a total of 813,702 unique reads, representing 11,691,441 high-quality total reads of between 1 and 5,731,905 copies.
