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'I said, "Bob, it looks like a total copy of Playboy!"' says Bradford.
Measurement of the intensity of hybridization signal indicated consistently a total copy number of between 50 to 80, which falls short of the estimated several hundreds copies of rDNA in mouse [35].
This gave a total copy number in each sample of 10 000 per μL.
ATon-II has a total copy number of 317, among which three elements have more than 10 identical copies (84, 15 and 12).
This results in a total copy number of 122 full-length IS elements that are evenly spread across the A. asiaticus genome [ 34].
Here we call a total copy number the total number of allelic copies over a diplotype (a pair of haplotypes) at an ICN site.
Similar(48)
However, a minority had an odd total copy number of AMY1 in association with deletions or duplications of AMY2 genes, and the frequencies of different AMY2 variants differed markedly between population groups (Supplementary Material, Fig. S4).
In other words, interpretation of the classical R0t results with correct parameters led to an estimated total copy number of ~31,000, which is in good agreement with the estimate from GATC-PCR or ~36,000.
We then have for each SNP locus a measurement of (total) copy number (logR) as well as the B allele frequency (BAF).
The data identified individuals with AMY2A and/or AMY2B variation, which was associated with an odd diploid total copy number of AMY1, in contrast to the majority of individuals with even copy numbers (Fig. 2).
With just four systems and a rolling backup strategy, you can end up with more than a million total copies.
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