Exact(27)
An aliquot of 25 μl of 2 mg/ml fluorescamine was added to a total assay volume of 100-μl diluted liposome suspension.
With its proven performance as a "total assay," the CUPRAC reagent is an attractive candidate as a derivatization reagent in an HPLC post-column instrumental setup.
Thus, 10 min for rapid DNA extraction followed by LAMP combined with LFD detection resulted in a total assay time of approximately 50 min.
Using a sandwich type assay, gold nanoparticles coated silicon quartz sensor chips provided the lowest limit of detection of ca. 1 ng/mL in a total assay time of 25 min.
This approach achieves an average of >80% recovery of spiked tumour cells from 2 mL of whole blood in a total assay processing time of 50 min without multiple processing steps.
Using a sandwich type assay, the response was ca. twice higher and consequently the lowest measurable concentration dropped down to 7 ng ml−1 for a total assay time of 25 min.
Similar(33)
NO release from the treated PMNs was assayed using a total NO/Nitrite/Nitrate assay kit (R&D Systems), which converts the nitrate into nitrite using nitrate reductase.
Aliquots of supernatants were subjected to a total protein assay using a BCA assay kit (Pierce , USA.
By using human recombinant NF155 and cell-based FCM assays, the specificity of our cell-based anti-NF155 antibody assay to CIDP was 98.9% based on a total IgG assay and 100% based on IgG4 subclass assay.
Real-time PCR assays were comprised of two independent reactions: a total quantification assay for quantification of total input DNA and an HM assay [ 21] for quantification of methylated target template.
The 25-OHD level was tested by the LIAISON 25-OH Vitamin D TOTAL Assay (DiaSorine), a competitive two-step chemiluminescence assay that measures both the D2 and D3 25-OHD metabolites.
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