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By summing up the scores a total amplification score is obtained.
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Thus total amplification A = A1*A2 = 1500.
Basically, the square-root impedance amplification is a first-order approximation of the total amplification derived by wave-calculation solutions.
The superior sensitivity of a total RNA amplification prior to qPCR is demonstrated, in fact about 12% more T-UCRs were reliably and reproducibly quantified by the SPIA™ system than using reverse transcriptase of total RNA, independently of the cell line.
Two-Temperature LATE-PCR does not require an external reference sequence and corrects for variations in total amplification product yield among replicate PCR samples by normalizing each sample to itself using mismatch-tolerant probes.
Second, in the present model, although based on the metabotropic pathway, the amplification from OR to PLC effectors accounts for only 3.5% of the total amplification (Fig. 7; [49]).
Total amplification of each multiplex reaction was kept below saturation levels to allow the amplifier to remain within the exponential range of the amplification curve and to provide semi-quantitative data.
The NBT approach is also limited in total amplification by non-specifically generated signal (Adams, 1992).
Increases in total amplification time increased the overall assay sensitivity, without the typical corresponding increase in non-specific signal.
When analysed per centre, the total amplification rates of all PBs were 97% in Bonn (245/252) and 92% in Bologna (183/200, P< 0.025) (Table II).
LATE-PCR used in combination with mismatched-tolerant probes is ideally suited for this approach because these technologies permit determination of both probe-specific and total amplification products in individual reactions.
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