Exact(6)
Samples were suspended in 50 mM phosphate buffer containing 0.5% hexadecyltrimethylammonium bromide (pH = 6.0) at a tissue concentration of 50 mg/mL.
We then resuspended the microsomal pellet in incubation medium at a tissue concentration of 1 g/mL and determined protein concentrations of both fractions using a bicinchoninic acid protein assay kit (Pierce, Rockford, IL).
The testicular tissue was homogenized in ice-cold 0.1 mol Tris Hcl buffer, pH 7.4 at a tissue concentration of 10%/ml and then the homogenized mixture was centrifuged at 10,000 × g for 30 min at 4°C.
Treatment with 0.5% ethanol, a tissue concentration of 19 mM (88 mg/dl), from 10 hpf to 5 dpf caused neurocranial defects in 3% and 13% of wild types and heterozygotes, respectively (Fig. 1G).
Cerium bone marrow content was not determined in our inhalation studies, but a tissue concentration of 6.61 ng/g bone marrow was reported 7 days after intratracheal instillation of 1 mg/kg of neutron-activated Ceria NM-212 (Molina et al. 2014).
At tissue levels above 10−4 M, ROS including O2 are cytotoxic and may act as cell aging signals [ 22, 48]; below a tissue concentration of 10−5 M, ROS promote cell proliferation and tissue repair (granulation, neo-angiogenesis and epithelialisation) [ 22].
Similar(54)
Frozen tissue samples were weighed and homogenised in phosphate buffered saline (P5493, Sigma-Aldrich, Dorset, UK) containing 2% sarkosyl (N-laurylsarcosinate 61745, Sigma-Aldrich) using a buffer volume that gave a final tissue concentration of 10% (wt/vol).
Frozen tissue samples were weighed and homogenised in phosphate buffered saline containing 2% N-lauroylsarcosine, to a final tissue concentration of 10% (wt/vol) using a Fastprep machine (MP Biomedical, CA, USA) as described by us previously [ 12].
However, these chelates cause low sensitivity and, thus, requiring a high tissue concentration of the contrast agent to be effective for MR imaging.
Livers were weighed and homogenized in a buffer (pH 7.5) containing 250 mM sucrose, 1 mM EDTA and 10 mM Tris, with a final tissue concentration of 5%. 2 µl of plasma or liver homogenate was used to determine TG.
Direct measurements of residual halothane in whole flies studied under equilibrium conditions also show a greater tissue concentration of halothane at the EC50 for induction than at the EC50 for emergence.
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