Figure 1 displays a SEM tilted image of a sample irradiated with a very low electronic dose (40 mC/cm2) and immersed in a pure PBS solution for 1 h.
Figure 2 shows a SEM tilted image of PS irradiated in the same conditions with that of the one displayed in Figure 1 but immersed in a 15-μM BSA-containing buffer solution.
To evaluate the transposition of the anterior superior oblique insertion as a treatment for cyclovertical diplopia accompanied by an awareness of tilted image perceived with the affected eye induced from limited macular translocation (LMT).
The two tilted image series were reconstructed into a single 3D density image stack using IMOD tomogram reconstruction software [ 22, 23].
The microscopist is obliged to keep the imaging area centered during tilting as well as to maintain accurate focus in the tilted image while minimizing the overall electron dose, a challenging and time consuming process.
The system includes a feature for montaging untilted images to ensure that all of the particles in the tilted image may be used in the reconstruction.
As the tilted image indicates, the inverted pyramidal pore arrays were reconsidered as ordered silicon pyramid arrays.
The dose for each tilted image was about 1 e/Å2, and the defocus was set to 3 μm.
Corresponding tilted images were centred only using an iterative method of custom masking.
Guided by the results obtained from this analysis, we then developed a statistical video processing procedure that does not depend on tilted images.
