The phrase "a threshold was established" is correct and usable in written English.
It can be used in contexts where a limit, boundary, or standard has been set or defined, often in discussions about policies, research, or measurements.
Example: "In the study, a threshold was established to determine the minimum level of exposure required for significant effects."
Alternatives: "a limit was set" or "a benchmark was created."
A threshold was established whereby the ability of a solution to vitrify could be determined by calculation.
For the identification of altered (gained or lost) DNA regions, a threshold was established based on the changes observed in the log2 CN values (fluorescence intensity ratio) of sequential tumour DNA segments found for each individual.
Through these control experiments a threshold was established at a gray scale value of 75.
For each digitalized image a frequency histogram of fluorescence intensity was generated: in this histogram a threshold was established to eliminate non-specific fluorescence, pixels with fluorescence intensity above the threshold were considered specific for SERT-IR.
Images were transformed into black and white, and a threshold was established to educate the program to identify positive staining of either nuclei or membrane with each antibody specifically.
Values were expressed as the number of median-adjusted standard deviations (Z-score value) and a threshold was established (Z-score < −2) to identify miRNAs that caused a significant reduction of luciferase expression when cotransfected with psiCHECK2-Cbx7-3′UTR. miRNas with a Z-score value lower than −1 when cotransfected with empty psiCHECK were discarded.
The peaks in the fingerprints are ranked according to the areas of the peak prominences, and a threshold is established to discriminate between the significant peaks and those that are irreproducible.
Four images per well were taken using 10× magnification with a numerical aperture of 0.25 and a camera binning of 2 × 2. A manual threshold was established by comparison of the total nuclear intensity in the positive control and the total intensity obtained in cells stained with the secondary antibody only (unspecific staining) and/or the vehicle control (DMSO).
Prior to exposure, a criterion threshold was established with recovery time being measured for five multiples of criterion threshold.
A minimal threshold was established by counting at least 1000 cells per sample and the results were expressed as a percentage of the total cell population.
A common threshold was established across all array cards for each miRNA, expression levels were normalized to MammU6 and average delta-Ct was used in downstream analyses.
