Glycerol content in the supernatant was determined using a nicotinamide adenine dinucleotide (NAD+) coupled enzymatic test kit with photometric quantification (Boehringer-Mannheim/R-Biopharm, Darmstadt, Germany) by downscaling to a twentieth proportion of the volumes listed in the supplier's manual and quantification via glycerol standard curves in a microtiter plate.
Just 7percentt of those over 65 reported problems in obtaining or paying for health care, a third the proportion of younger adults.
Combined with strategies such as car-free residential developments, where car parking is pushed to the periphery rather than outside homes, such planning has allowed many cities in the Netherlands, Denmark and Germany to increase by a third the proportion of commuters who cycle.
A third, considerable proportion (15%) did not report on the use of measures specifically within a pediatric population.
In addition, SNA lectin can also be detected in a one-third proportion of K14+ cells (ratio of SNA+/K14+ cells, 0.36±0.09, n = 284) (Fig. 2B).
"I think it is a tragedy of the first proportion that a private corporation can be subjected to what I would characterise as a shakedown, in this case, a $20bn shakedown," Barton said at the time.
—Ken Salazar, Secretary of the Interior "I think it is a tragedy of the first proportion that a private corporation can be subjected to what I would characterize as a shakedown in this case, a twenty-billion-dollar shakedown".
— Ken Salazar, Secretary of the Interior "I think it is a tragedy of the first proportion that a private corporation can be subjected to what I would characterize as a shakedown — in this case, a twenty-billion-dollar shakedown".
"I think it is a tragedy of the first proportion that a private corporation can be subjected to what I would characterize as a shakedown -- in this case a $20 billion shakedown".
He called it — the escrow fund, not the oil spill — "a tragedy of the first proportion".
