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After a washing step with buffer A, the gel pieces were dried in a vacuum centrifuge, and rehydrated at 4°C in a digestion buffer (50 mM NH4HCO3, 5 mM CaCl2) containing 25 ng/µl trypsin.
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As a result, the gel exhibits a periodical swelling deswelling change.
(A) The gel-filtration was performed using a Superose 6 column (10/3004 GL, GE Healthcare).
For highest activity as a desiccant, the gel is not completely dehydrated but is left with a small percentage of combined water.
As a moisturiser, the gel smoothes away to nothing.
As a result, the gel expands slightly, but the polymer chains themselves remain intact.
In a month, the gel has completely disintegrated.
(a) Photograph of the gel in a quartz tube before combustion.
Take a tablespoon of the gel anytime you experience a coughing spasm.
Even on a cinder block, the gel matches the comfort of a down-filled settee.
After a final dehydration, the gel plugs were dried in a speed vac.
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