From these alignments, a phylogenetic tree was constructed using MEGA 4.0 program [ 51], according to the neighbor-joining method with a bootstrap test calculated with 1000 replicates and a poisson correction model.
p-value of a t test calculated for the last time point is indicated.
The J-index i.e., the over all measure of reliability of a diagnostic test calculated as (TPxTN - FPxFN / (TPxTN - FPxFN /
Different conditions were compared using a two-tailed Mann-Whitney test (calculated by PRISM software) with a p<0.05 being considered significant.
In order to detect if these two genes have evolved neutrally, we performed a McDonald Kreitman test calculating the neutrality index (NI) [ 48].
We compared the coefficients from the various codes with each other using a kappa homogeneity test, calculating the value of the global kappa and its 95% CI.
Components of variation were estimated from an analysis of variance (ANOVA) model, using an F-test calculated from proc mixed within SAS.
The likelihood ratio test calculates a P-value by assuming a χ distribution with one degree of freedom for the statistic, − 2 ln Λ.
The Shapiro-Wilk test calculates a W statistic that tests whether a random sample of size n comes from a normal distribution.
This test calculates a global allele frequency based on the prevalence of the disease in order to calculate expected frequencies of each genotype in cases and controls separately, and a permutation test is used to determine the significance.
