Sentence examples for a template of approximately from inspiring English sources
Provision to design primer for selected STR locus is provided with a template of approximately 1000 base pairs by selecting upto 500 base pairs of both flanking regions.
Primer for selected STR locus may be designed with a template of approximately 1000 base pairs by selecting upto 500 base pairs of both flanking regions.
PCR reactions were of a total volume of 15 μl or 50 μl, with a template of approximately 50 ng genomic DNA, 1 ng Plasmid DNA or 50 ng cDNA, 5 10 pMoles of the forward and reverse PCR primers, 0.1 mM of each dNTP (Boehringer Mannheim), 1.5 2.5 mM MgCl2, and 1U of TaqGold DNA polymerase (PE Biosciences).
Our extensive optimization experiments indicated that a template of moderately high quality would require approximately 20 cycles of multiplex pre-amplification, another 35 cycles of singleplex amplification on the Fluidigm Access Array, and finally an additional 15 cycles of barcoding PCR, for a total of 70 cycles of amplification.
Primers were designed to specifically amplify templates of approximately 90-130 nucleoverlappinglapping exon boundaries of 3' terminal exons using the Primer-Blast software http://www.ncbi.nlm.nih.gov/tools/primer-blast/.
Consistent with this, the structural model of the minimal open promoter complex constructed from the pol II TFIIB crystal structure defines a template strand path of approximately 30 residues from the TATA box to the catalytic site [ref 9] and [ref 10].
High-resolution transmission electron microscope (HRTEM) and field emission scanning electron microscope (FESEM) images have shown the replication of the porous template diameter of approximately 200 nm with a superior incorporation of both VOPcPhO and PC71BM.
Figure 1a shows a well-ordered array of circularly shaped holes with a pore diameter of 67 nm. Figure 1b is the cross section of this PAA template, with a thickness of approximately 200 nm.
The addition of NU7441 or KU-0060648 to cells following introduction of the Cas9/ sgGFP editing system and the ΔGFP repair template caused a decrease of approximately 40%% in NHEJ events which was accompanied by an approximately two-fold stimulation in HDR (Fig. 1f, g).
