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Although there is no crystal structure for human PABP1 RRM4, a solution NMR structure for RRM4 of human tPABP (PABPc3), which differs in only one of 71 amino acids, is derived (PDB code 2D9P) allowing its use as a template for predicting the structure of human PABP1 RRM4.
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Table 1 provides an example overall process template for predicting an endpoint for a chemical structure, which the ToxPredict application described later on is based on.
Table 1 Overall Use Case process template for predicting an endpoint for a chemical structure Activity Name: Overall Use Case - Given a chemical structure, predict endpoints.
Our research has evidenced that the structure of archeal (Natronomonas pharaonis) phototaxis protein rhodopsin II (PDB code: 1GU8) presented similar lengths of helices and loops between the helices respect to ADIPOR1 and, thus, suggested that this protein was the correct template for predicting the three-dimensional structure of ADIPOR1 by comparative modelling strategy.
We verified the Pfam results by visual inspection of the sequences defining a LRR motif as LxxLxLxx, or variations of it where L is substituted with I, V, or A. To track the molecular evolution of the LRR-domain, OsLRR-PSR was used as a template for comparison to the predicted domains of IRI-like sequences.
2 ng of the extracted DNA was next used as a template for PCR for the different predicted S/MAR sequences in Giardia.
Further, we have confirmed the validity of the HTP structure as a template for structure-based drug design by predicting binding affinities for TPI and other known HTP inhibitors using in silico docking techniques.
Through this study, an ANN was designed to present a template that is capable for predicting output data (coercivity) according to input data (pH, oxidative and reductive times, oxidative and reductive voltages, and off-time).
The results indicate that choosing a good structural template is critical for predicting the binding energy accurately.
We describe here a new template-based method for predicting the structure of protein loops, a complex, yet critical, problem that is considered one of the bottlenecks for accurately predicting protein structures.
A pair of predicted binding sites on a transcript define a region that serves as a template for synthesizing a dsRNA for siRNA production [ 8– 11].
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