Sentence examples for a template for complementary from inspiring English sources
In the genetic system a parental DNA strand acts as a template for complementary activated nucleotides.
Total RNA from human and mouse keratinocytes were extracted with TRIzol reagent (Invitrogen) and were employed as a template for complementary DNA (cDNA) synthesis performed with SuperScript Vilo (Invitrogen) according to manufacturer's instructions.
When the vector is introduced into a bacterial cell and replicates, the mutated strand will act as a template for a complementary strand that will also be mutant, and thus a fully mutant molecule is obtained.
RISC uses miRNA as a template for recognizing complementary transcripts.
It is now known that DNA replication is semiconservative: each of the strands of the double stranded DNA molecule is used as a template for constructing a complementary strand, and the replication process results in two DNA molecules, each containing one of the original strands and one newly constructed strand.
After cooling the RNA samples on ice, 20 µL of each sample was used as a template for first strand complementary DNA (cDNA) synthesis using the First Strand cDNA synthesis kit (GE Healthcare Life Sciences, QC, Canada) according to the manufacturer's recommended procedure.
Total RNA was isolated from cell lines, tumour cells in clinical samples, and from PBMCs using Trizol reagent (Invitrogen, Carlsbad, CA, USA), and was used as a template for the synthesis of complementary DNA by RT using random primers (Invitrogen).
The second proposed mechanism, known as semiconservative replication is when each strand of the parental DNA acts as a template for a second newly-synthesized complementary strand, which then combines with the original strand to form a DNA molecule.
The aberrant transcript becomes a template for RDR6, which synthesizes the complementary strand and produces dsRNA.
The precipitated RNA was used as a template for primer extension using oligomers complementary to the U1, U2, and U4 snRNAs.
Each strand is used as a template for the construction of a complementary strand.
