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The enzyme, known as Taq DNA polymerase, is used in the polymerase chain reaction, a technique to amplify minuscule amounts of DNA for use in medical diagnosis and DNA fingerprinting.
We fully characterized Stylonychia lemnae macronuclear genome encoded H3 variants using degenerate oligonucleotides in combination with telomere suppression PCR (TSP), a technique to amplify the 5'- or 3'-ends of Stylonychia nanochromosomes including their telomeric sequences [ 45].
Subsequently we used this sequence information to design Stylonychia-specific primers for the characterization of the whole nanochromosome by telomere suppression PCR (TSP), a technique to amplify the 5′- or 3′-ends of Stylonychia nanochromosomes including their telomeric sequences (Curtis and Landweber, 1999), eventually resulting in a complete nanochromosomal sequence.
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One-step nucleic acid amplification (OSNA) is a novel technique to amplify mRNA directly from tissue lysates in an isothermal manner and has been developed to provide quantitative measurement of cytokeratin 19 (CK19) mRNA expression.
Thus, application of PNA-PCR clamping was considered to be effective and is a useful technique to amplify the rhizobacterial SSU rRNA genes from selectively extracted DNA containing plant mitochondria and plastid genes.
Our results suggest that manipulating the VOCC and attenuating the opening of the calcium channels may be an effective technique to amplify matrix production via mechanical stimulation which may be applied to bone tissue engineering and potentially engineering of other load-bearing connective tissues.
Watched by a small group of police officers, the assembled discussed the future of their protest, using the "human microphone" technique to amplify a speaker's voice for those at the back.
Herein we present a universal high-throughput, rapid PCR-based technique to amplify, sequence and assemble plastid genome sequence from diverse species in a short time and at reasonable cost from total plant DNA, using the large inverted repeat region from strawberry and peach as proof of concept.
From the gene sequence we designed oligonucleotide primers to use in the polymerase chain reaction technique to amplify the appropriate exons in DNA from individuals with three variants of hereditary elliptocytosis characterized by the presence of abnormal alpha I spectrin peptides, 46-50 and 65-68 kD in size, in partial tryptic digests of spectrin.
But the most telling part of the day came at its end, when the families of victims, including Ramarley Graham, Alberta Spruill, and others, stood at the center of thousands, using the mic check technique to amplify their voices.
These are offered by companies such as Clontech, Sigma, Miltenyi Biotec, and NuGEN, and use a variety of techniques to amplify RNA.
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CEO of Professional Science Editing for Scientists @ prosciediting.com