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To demonstrate a therapeutic advantage of a targeted version of IL-10, compared with the untargeted cytokine, the two fusion proteins L19 IL-10 L19 IL-1010–IL-10 were investigandd in tHyHEL10 IL-10 HyHEL10 IL-10
To test the therapeutic potential of a targeted version of the anti-inflammatory cytokine IL-10, we produced the fusion protein between single-chain Fv(L19) and IL-10 using a gene-fusion approach (L19 IL10; Figure 3a,b).
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You can see how this sort of framework could potentially prove to be a useful way for people to interact with the whole web at large, as a more targeted version of a search portal.
This comparative in silico mapping approach is the targeted version of the COMPASS mapping procedure (e.g., [ 31]), which utilizes the human-cattle comparative mapping knowledge for predictive positioning of unknown bovine ESTs on the bovine genome.
YouTube Kids is a kid-targeted version of YouTube that features curated, ad-supported TV shows, music, educational videos and user-created content.
To do this we utilized the UAS/GAL4 system to specifically express transgenic constructs encoding either wild type, dominant negative (a truncated version of C3G lacking the C-terminal CDC25 catalytic domain) or constitutively activated C3G (a membrane-targeted version of C3G) in somatic muscles [13].
Here we report the development and characterization of MitoC, a mitochondria-targeted version of the hydrophilic antioxidant ascorbate.
DOI: http://dx.doi.org/10.7554/eLife.09143.024 To test this conclusion within undisrupted parasites, we created a transgenic parasite line expressing an apicoplast-targeted version of the uroporphyrinogen III methyltransferase (cobA) protein from Propionibacterium freudenreichii to serve as a biosensor of heme biosynthesis pathway activity within the parasite apicoplast.
Although transgenic tobacco plants expressing this construct were stunted, those expressing an ER-targeted version of murine IL-10 did not show an obvious morphological phenotype [ 14].
Finally, all of these effects were efficiently rescued by the additional expression of an ER-targeted version of Rhomboid-1, but only slightly rescued by high levels of wild-type Rhomboid-1, which is located in the Golgi apparatus.
BY-2 suspension cell lines constitutively expressing viral interleukin 10 (vIL-10) were produced by transforming wild-type cells with construct pTRAkt_ER-vIL-10, which encodes an ER-targeted version of the protein under the transcriptional control of the double enhanced CaMV 35 S promoter [ 14].
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