Sentence examples similar to a targeted sequencing protocol from inspiring English sources

Orthologous Otopetrin sequences were generated by a targeted sequencing approach, or identified through tBlastn searches of available whole-genome sequences.

Transgenic animals were synchronised and subject to the acute RNAi protocol with scoring occurring at 24 hours post introduction to RNAi clone or a control clone lacking a targeted sequence.

To overcome this PE read imbalance in targeted sequencing, we modified the standard PE sequencing protocol of reading the forward and reverse strand of a DNA template during one PE read.

We show that our approach maximizes coverage uniformity across increasingly larger capture products and is fully compatible with existing library preparation protocols used by a variety of multiplex targeted sequencing methods.

A popular approach for targeted sequencing is sequence capture (e.g., Crosby and Criddle 2007).

The rPE strategy is applicable to library preparation protocols of other targeted sequencing methods.

Restriction-site associated DNA (RAD) sequencing is a powerful new method for targeted sequencing across the genomes of many individuals.

Here, we describe a targeted amplicon sequencing (TAS) approach capitalizing on next-gen capacity to sequence large numbers of targeted gene regions from a large number of samples.

The drive to produce increasingly compact, cost-effective and time-efficient sequencing platforms has also resulted in products that can be utilised more readily for targeted sequencing of genes with easier sample preparation protocols, shorter run times and simpler data analysis (Desai and Jere, 2012).

Paired-end 100-bp sequencing was performed on an Illumina HiSeq 4000 with targeted sequencing coverage of 50 M reads.

These libraries were sequenced on the Illumina HiSeq 1500 using a 2 × 106-nt paired-end sequencing protocol, yielding 84.7 M paired-end reads.