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The exons and adjacent 20 bp of the genes in the region of interest were selected from the EquCab2.0 genome (Broad Institute, Cambridge, MA, USA) to design a Sure-Select slide (Agilent Technologies).
Exome capture was performed using the Sure Select Human All Exon 50 Mb System (Agilent Technologies, Santa Clara, CA).
In brief, the sample was prepared and enrichment was carried out according to Agilent's Sure Select Protocol Version 1.2.
Subjects from the replication panel were further genotyped by sequence enrichment of the 12p13.2 locus on Sure Select arrays (Agilent Inc., Santa Clara, CA, USA) followed by HiSEQ (Illumina Inc).
If you are not sure, select MP3 encoding.
Targeted enrichment sequencing was conducted based on the Sure Select-XT Target enrichment system (Illumina paired end and multiplexed sequencing library by Agilent Technologies).
Sure, a select few have come close.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com