The cell pellet was resuspended in 100 ml of 20 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM EDTA (Buffer A) supplemented with 1% PMSF and sonicated on ice.
The cells were resuspended in 20 mM Tris HCl, 50 mM NaCl, pH 7.4 (buffer A), supplemented with a Complete® protease inhibitor mix (Roche, Basel, Switzerland) and disrupted on ice by sonification.
This means that the token line of Mary exists in the word bank of agent B, though without a supplemented name proplet pointing at the referent proplet daughter.
The supernatant was purified by Ni Sepharose (GE) affinity chromatography equilibrated with buffer A. Proteins were eluted using buffer A supplemented with 250 mmol/L imidazole.
In our department, the cost for a tomosynthesis examination, including the automatically generated AP radiograph and a supplemented lateral radiograph, is equivalent to 54€, which is about 10% more than for a chest radiograph (48€).
25 g/kg calcium chloride was used for preparing A, supplemented with 200 g/kg trehalose for A-T, AP-T, and AAG-T beads and with 5 g/kg of chitosan for A-TCh.
In another study from Bangladesh 250 g/L of green (unripe) banana (eqv. to two fruits) or 2 g pectin/kg food was compared to a supplemented rice diet in children suffering from persistent diarrhoea [21].
We questioned whether the change in proteinuria in response to a supplemented very low protein diet (SVLPD), which is known to reduce proteinuria, could function as a marker of the potential renoprotective effect of an SVLPD.
