Delhi Police also filed Tuesday a supplementary charge sheet, which included the postmortem of the rape victim, who died in a Singapore hospital on Dec. 29.
The total number of species detected for each of the AS/AR pairs was mostly the same (Supplementary Sheet S1).
Based upon these differences we sorted out a list of lipid species that show modest to robust variations among the AR isolates (Supplementary Sheet S3).
The loading scores associated with the principal component 1, 2 and 3 are summarized in the Supplementary Sheet S4 (Supplementary Figure S5B).
In contrast, those pairs where azole resistance was due to CaMDR1 over-expression, ∼66 127 lipid species were significantly different (Supplementary Sheet S3).
The highest and lowest loading values reflect the lipid species that are most important variable in the assignment of each principal component (Supplementary Sheet S4).
We performed PCA using the molecular species percentage composition of PGL + SL + SE (data in Supplementary Sheet S1) to identify and highlight the AR-specific, statistically significant lipid differences.
The 3-D PCA plot on all combined AS and AR datasets confirmed that all AS/AR pairs have unique lipid profiles as they did not segregate as different groups of isolates (Figure 6 and Supplementary Sheet S4).
By mass spectrometric analysis of the extracts of AS and AR pairs, we detected molecular lipid species belonging to five major lipid groups (PGL, SL, SE, DAG and TAG; see Supplementary Sheet S2).
The total lipids (PGLs, SLs and SEs) were quantified and lipid content (as total normalized mass spectral signal of PGL + SL + SE) was found to range between to 453 to 1116 nmol per mg dry lipid weight of AS and AR isolates (Supplementary Sheet S1).
For example, out of 242 lipid species of 18 major lipid classes (PGL + SL + SE), among CaCDR1 attributed pairs, only the pair DSY544/DSY775 showed significant differences in ∼128 lipid species, while other pairs did not show much variation in lipid species (∼18 70 species) (Supplementary Sheet S3).
