However, by enhancing the training with supplementary labeled data from a related source domain, the model's ability to classify target instances can be improved.
The aim of this study was to develop whole-mount tissue processing methods for 3D in situ visualization of the microvasculature that were also compatible with supplementary labeling for other structures of interest in the tissue microenvironment.
We tethered tetR-EYFP fusion proteins for 2 h by controlling the presence and absence of doxycyline, and detected new CENP-A assembly using a SNAP-CENP-A pulse labeling technique (Supplementary Figure S13A, B).
To assess the influence of the N-terminal fluorescein dye on binding, we tested a subset of unlabeled peptides, MS1, MS2, Bim, and NoxaA in competition with a fluorescently labeled Bim variant (Supplementary Figure 2).
We also simulated local [Ca2+] change using a real immunogold distribution directly taken from the replica labelling (Supplementary Fig. 1a), finding a similar Ca2+ waveform (Supplementary Fig. 2c, d).
Supplementary Fig. 2 (A) Double labeling with anti-collagen III (col III, green) antibody, used as a marker of fibrosis, and anti-α6 chain antibody on transversal sections of MDC1A muscle biopsy showed increased expression of the α6 chain (red) in fibrotic areas.
The development of renal tubules was largely normal judged by calbindin-D28K labeled ureteric bud (UB) and E-cadherin labeling (supplementary material Fig. S3B).
The gt_indel_2 and gt_indel_7 primer sets included an M13 site on the left primer to enable fluorescent labeling in a 3-primer reaction, while the gt_indel_9 left primer was directly fluorescently labeled (Supplementary file 1).
The mismatches that occurred in the stem (potentially influencing the secondary structure) and loop were then labeled (supplementary fig. S1, Supplementary Material online, shows a demonstration).
As in the CALM-PD study, if symptoms were not adequately controlled by the assigned study medication, patients could receive supplementary LD, administered in an open-label fashion.
Proliferation was not significantly altered, as assessed by BrdU pulse labeling (Supplementary Fig. S2).
