Of particular importance is a so-called LPXP motif within the receptor subunit that forms a minor yet crucial supplementary interaction with the Cullin NTD [ 21].
Therefore, we decided to restrict all model fits and tests to 32 out of the 36 data points where the repulsion effect is due to inhibitory interactions and not affected by yet unknown supplementary interactions (discarding surround angular deviations of ±10° and ±20°, see Fig. 5, grey data points).
With limited experimentally validated miRNA-target interactions, expression profiles can only serve as a supplementary role in finding interactions between miRNAs and mRNAs.
For this reason, we utilize the mood of music that a user is listening to, as a supplementary indicator for affective interaction.
Both Dpb11-WG700,701AA anDpb11-W700A0A were unable to interact with Mec1 Ddc2 and Dpb11 YG735,736AA showed a reduced interaction (Supplementary Figure S2E).
A limited Co-IP screen was performed with Arf6 and several Rabs in Xenopus embryos, and Xenopus TBC1d24 interacted with Rab5 and Rab35, but Arf6 showed only a weak interaction (Supplementary Fig. 3a).
In the 2812 participants with available Tanner stage data, adjustment for Tanner stage led to a 39% attenuation in regression coefficient for the association between birth weight and BMDC, and there was no longer evidence of a gender interaction (Supplementary Table 4).
However, intense NOE connectivities from both the H1′ and H8 protons of the preceding A39 to the H3 proton of U68 show that A39 is sequestered in a reverse Hoogsteen interaction (Supplementary Fig. 2).
Stability analysis using PISA (Protein Interactions, Surfaces and Assemblies) software calculates the HAdV-D26 and HAdV-D48 trimers to have >20% lower interface energy than that of HAdV-C5, indicative of a more stable interaction (Supplementary Figure 2A) between the monomers of the HAdV-D26 and HAdV-D48 fiber-knobs.
These gene products directly interact in a protein interaction network (Supplementary Figure 7B), suggesting functional associations.
Edge weights w e) depend on our confidence that a specific interaction exists (Supplementary Table S1) so that more confident pathways have higher weights (Gitter et al., 2011).
