Since it is not possible to describe all of the observed changes, a supplementary table (supp_table_1_cluster) is included for further analysis.
As a supplementary effort to fight climate change, some of the reinforcements obtained by the EP (+€4 million for LIFE, +€8 million for sustainable transport in Horizon 2020) aim at inching closer to the target of 20% of climate-relevant spending in the EU budget for the 2014-2020 period.
Adjusting for whether or not a supplementary interview was performed did not change the results of the logistic regression analysis.
Furthermore, the bioinformatic prediction packages PolyPhen2, MutationTaster, Sift, Blosum62, Provean and MutPred consistently predicted this to be a likely pathogenic change (Supplementary Material, Table S1).
P-values were calculated based on comparison between the reshuffling-based and observed LR scores (a stepwise change test, Supplementary Note 1).
From the initial 1890 genes, we identified the top genes (i.e., 370 up-regulated and 368 downregulated) with a fold change >1.5 (Supplementary Data 1) in either direction (cutoff log 2 FC < 0.5845 < ).
The gene list was sorted based on a 1.5-fold change cutoff (Supplementary Table 2, see section on supplementary data given at the end of this article) and analyzed by cluster and tree-view analyses and subjected to functional analysis using Ingenuity Pathways Software.
During the optimization of the device, incorporation of 2 mol% Cs was found to further enhance the performance and reproducibility of the devices without a noticeable change in Eg (Supplementary Figs. 8 12, see Supplementary Notes 1, 2 and 3 for additional discussion on the optimization process and impacts of 2 mol% Cs).
Interestingly, for the mesophiles group 19 specific metrics did show a significant change in correlation (supplementary fig. S1, Supplementary Material online).
Strikingly, when live cells stably expressing CAV1 EGFP at low levels were imaged (Supplementary Figure S3C), a dramatic change in caveolar dynamics was observed after EHD2 depletion (Supplementary Movie S1).
Mutation S159R was incorporated in all the combination variants, showing a high affinity ratio change (Supplementary Table 1).
