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All alignments were concatenated in a super alignment of 82,950 base pairs.
A super alignment of the fungal proteomes was constructed with the Hal pipeline [ 75], allowing for no missing data.
Their alignments, as constructed in the phylome, were then concatenated to form a super alignment that contained 1,479,842 amino acid positions.
Within the provided additional file is a super alignment including many major families within the classical ubiquitin superfamily, as well as individual alignments of all newly-discovered β-GF domains.
For the analysis of phylogenetic relationships of the four A. pullulans varieties and related species, a super alignment of the selected fungal proteomes was constructed with the Hal pipeline [ 175], allowing for no missing data.
Similar(55)
The 218 single alignments were concatenated to form a super-alignment with 192,102 aa positions.
This resulted in a super-alignment with 135,360 aa positions (Additional file 2), which was used for initial maximum likelihood (ML) and Bayesian tree reconstructions.
Another approach of phylogenetic reconstruction was concatenating all alignments into a super-alignment of the total length of 390.024 amino acid residues.
These 100-protein alignments were concatenated into a single super alignment, which contained 93,210 amino acid positions, and analyzed using RAxML (Stamatakis 2006) with the best model of amino acid substitution assigned to each protein partition; branch support was estimated from 100 bootstrap partitions with the rapid bootstrap option in RAxML.
Core sequences from each species are concatenated and the super alignment is used in phylogenomic analysis.
For each species, back alignments of CEGs or protein alignments were concatenated in the same order to generate super alignments.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com