Although none of these maize populations is fully resolved by markers, achievement of appropriately high marker density will fade as a major limitation, since transcript-derived markers have been shown to be reliable for generation of thousands of new markers per experiment [7], and suitable transcript profiling platforms exist for at least 14 crops as well as for all animal model systems.
As a result, they were selected as the most suitable transcripts for the normalization of the target genes of biological interest further analyzed by RT-qPCR.
Next-generation sequencing could greatly facilitate the identification of suitable transcripts leading to potentially useful promoters, and also of alternative splicing systems.
The properties of the recombinant fused version, in which the small subunit was followed by the large subunit with a 10 amino acid linker, resembled those of the native heteromeric enzyme in kinetics, product chain-length specificity, and architecture, and this form thus provided a suitable single gene transcript for biotechnological purposes.
The GeneChip® soybean Genome Array is a suitable platform for transcript profiling in common bean.
The GeneChip® Medicago Genome Array, developed for Medicago truncatula, is a suitable platform for transcript profiling in tetraploid alfalfa [ Medicago sativa (L). subsp. sativa].
To meet the criteria for a suitable reference gene, the transcript must be detected in all organs, developmental conditions and under a variety of stress conditions.
A list of differentially expressed transcripts was then created by first selecting any transcript with a suitable difference associated with a small false discovery rate (FDR), and further narrowed by selecting for transcripts with an estimated absolute raw fold change ≥1.25.
Transcript profiling and qRT-PCR data suggests that the soybean GeneChip is a suitable cross-species platform for transcript profiling in common bean when used in combination with the masking protocol described.
Previous research indicated that the Medicago GeneChip® is a suitable cross-species platform for transcript profiling in alfalfa [ 11, 12].
