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Domain 1 also works quite efficiently with full-length muNS, but not with muNS-Mi, and besides it is too large for a suitable tag.
Previously [25] we have demonstrated that the avian reovirus muNS IC domain is a suitable tag for targeting fused proteins to the inclusions formed by different muNS versions: muNS-Mi, GFP-muNS and GFP-muNS-Mi (Figure 1A and manuscript in preparation).
These protocols have yet to be applied to the analysis of genomic DNA due to the lack of a suitable tag such as the polyA tail.
These tags are then checked for secondary structure formation against all sense primers, and the first tag to have all dimerization free energies above -6 kcal/mol is output as a suitable tag.
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For instance, the His-tag may be replaced with a more suitable tag for the immobilization purpose, because we already know the His-tag in this position did not disrupt the protein folding and enzyme activity.
As weight increased by 1 g, the fish were 4.73 times more likely to survive PIT-tag implantation compared to the control fish with an estimated suitable tagging size at 1.1 g (TL: 39.29 ± 0.41 mm).
If you think pinning a suitable price tag on it and waiting for it to take the various app stores by storm is all that's needed, you could be in for a nasty surprise.
Protein Pilot automatically searches for a series of potential biological and sample preparation-induced modifications once a suitable sequence tag of 3-4 acids acids has been found within an MS/MS spectrum.
One suitable tag was manually selected and annotated to each segment for performance verification.
It is shown that plastic dart tags are the most suitable tag as there was 100% initial retention and long-term loss of 2% per annum.
Unfortunately, some genes already known to be associated with pigmentation were not selected due to reasons such as large gene size, lack of suitable tag SNPs within the gene, genes lying in high copy number regions, etc.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com