For the protein expressed in human embryonic kidney 293 cells, we could not obtain a sufficient amount of material for crystallization; therefore, we decided to work with the isolated RFM domain, which was predicted to be active based on analogous experiments with the Trypanosomal homolog43.
Strengths of the solutions were designed such that a reference method (RM) was able to obtain a sufficient amount of material on filter samples in 30 min cycles; the collected filters were subsequently analyzed in an analytical laboratory using certified methods.
These data provided a full description of the training setting and a sufficient amount of material on the behaviours of the actors to carry out interviews.
Clay separate samples were taken starting at 1042 m because that is the approximate depth at which clays become abundant enough in the groundmass to collect a sufficient amount of material for X-ray analysis.
When a sufficient amount of material is accumulated, planets or comets can be formed here".
In addition, they may not result in a sufficient amount of material for follow-up experiments.
Once a sufficient amount of material was withdrawn from the cell, the patch pipet was quickly removed from the slice and ∼1 mm of the distal tip broken off against the bottom of a 0.2 mL thin-walled PCR tube (USA Scientific, Inc., Ocala, CA).
Although 2 weeks was enough to produce a sufficient amount of leaching materials to be analyzed by IR spectroscopy, it is likely that all commercially available resin-based dental materials will continue to release components that may cause detrimental effects or alter cellular function in vitro even after 2 weeks of aging in artificial saliva.
This choice was done on the basis of the consideration that four parallel channels ensure that the pre-concentrator can be filled with a sufficient amount of adsorbent materials (Tenax-TA powder), while a cross section area of 0.8 mm2 allows a simple cavitands introduction.
For a sufficient amount of sediment material each sample from the open cores (SAT 7 and 10) represents sections of 5-10 cm from significant stratigraphic units.
However, the sixth fraction was collected after a longer elution time with the aim to cumulate a sufficient amount of humic material for good-quality NMR analysis (>5 mg).
