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Of the 26 patients who had a successful marker placement, 23 went on to have a good response to chemotherapy.
Expression plasmid encoding CD8 was also added (in pIRES, donated by Dr I Baró, University of Nantes, France) to be used as a successful marker of transfection.
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Two other phase-III adjuvant breast cancer trials, BETH and BEATRICE (Cameron et al, 2013; Slamon et al, 2013), have been negative, highlighting the limitations of bevacizumab for breast cancer in the absence of a successful efficacy marker.
Thus, the inclusion rate for 1500 and 3000 means the probability of successful marker validation when the code watchdog inspects a single packet and two sequential Ethernet packets, respectively.
A total of 53 out of 133 plants showed a 1.2 kb product using primers P1 and P2, indicating the occurrence of successful marker excision.
Successful marker assisted selection (MAS) programs utilizing the GT haplotypes for improving starch profiles have been reported (Tian et al. 2009; Lu et al. 2010).
Genotyping 720 of the MAGIC lines and eight founders returned successful marker scoring for 62,543 SNPs.
PCR analysis revealed a smaller fragment of expected size in two out of ten regenerated shoots consistent with successful marker excision.
Key design concerns for successful marker development are ensuring that the PCR primer sequences are specific for the target Indel locus and that the expected PCR product is within the range of resolution for a 4% agarose gel.
Where markers failed (for example, were found to be multi-copy), further markers were chosen from nearby sequences in an attempt to find successful markers.
Genetic mapping of successful markers was performed with Mapmaker v. 3.0 [ 56] and a minimum LOD of 3.0 and using the genetic map from Blair et al. [ 17].
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