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We report here a successful expression of the single-module cellobiohydrolase Cel7B from a thermophilic fungus Melanocarpus albomyces in Saccharomyces cerevisiae (Sc Cel7B).
DN3 thymocytes with a productively rearranged TCRβ locus and a successful expression of the pre-TCR complex pass the β selection checkpoint, downregulate CD25, and develop into thymocytes with a DN4 phenotype (CD25−CD44−).
Here, we were able to demethylate the SOX11 promoter in the ovarian cancer cell line ES-2, resulting in a successful expression of SOX11 mRNA and protein.
After a transfection of FLAG-E2 expression plasmid RT-PCR and western blot assays were conducted to ensure a successful expression of E2 protein in Huh7 cells.
Both transcriptional and translational levels of mkp-1 in H441GL cells were significantly up-regulated, indicating a successful expression of mkp-1 gene.
But a successful expression of small disulfide-rich peptides like the conotoxins has not been reported so far in such a strain.
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Periplasmic secretion using E. coli has proved a successful expression strategy for the functional folding of many disulfide-rich proteins e.g. antibody fragments [30].
Recently, successful expression of a mammalian optimized luciferase dimer in an HEK293 cell line has provided for the limited use of lux as a mammalian bioluminescent reporter system, although the addition of luciferin in a manner similar to firefly luciferase is still required [17].
More recently, Juillerat et al reported successful expression of a rosette-mediating PfEMP1 domain in E. coli as a fusion protein with maltose-binding protein, and showed that addition of the NTS to DBL1α resulted in soluble protein [52].
We further demonstrated the successful expression of a heterologous gene coding for green-fluorescent-protein by fluorescence measurement.
In this study, we demonstrated successful expression of a fusion protein consisting of Tsf and green fluorescence protein (GFP) on E. coli flocs.
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