A polycarbonate substrate featuring a honeycomb pattern of 1,024 hemi-spherical wells (1.3 mm diameter) was used to create a negative mould in flexible polydimethylsyloxane (PDMS, Sylgard 184, Dow Corning) using standard moulding techniques.
The former substrate featuring a stiffness of 1 GPa, which was approximately six orders of magnitude stiffer than the latter with stiffness of 1 kPa [ 28].
A thin-film microthermocouple array was lithographed on the silicon substrate, featuring N-type probes.
Skin explants cultured on substrate featuring 2 kPa stiffness exhibited the highest degree of full recovery (83.3%; n = 5 in 6) compared with other substrates featuring stiffness of 12 kPa (33.3%; n = 2 in 6) and 50 kPa (50%; n = 3 in 6) (Table 1).
Compared with the conventional PP with glass-cloth, a cost effective substrate featured with 20% thinner and 20% less package warpage deformation was gained.
Moreover, the dense coating obtained, with a supposed metallurgical bonding to the steel substrate, featured a packed columnar microstructure mixed with nano-sized particles.
Substrates featuring a 1,6- or 1,7-relationship between the silyl enol ether and the alkyne such as 181 undergo an exo-cyclization to form five- (182) or six-membered rings (183), respectively (Scheme 56).
To further characterize the mode of action of Eng2, the products formed with linear laminarioligosaccharide substrates featuring different degrees of polymerization (DP) were analyzed using HPAEC.
Further, the employment of [1 1]-orienteded CdTe or CdSe substrates featured an epitaxial growth of polycrystalline ZnTe films as well as an improved Zn/Te atomic ratio compared to the metallic substrates.
In contrast to RhoE phosphorylation sites, these optimised substrates feature multiple basic residues N-terminal to the phosphoacceptor site (Supplementary Figure S4B).
Walmacq C, Rahmouni AR, Boudvillain M. Testing the steric exclusion model for hexameric helicases: substrate features that alter RNA-DNA unwinding by the transcription termination factor Rho. Biochemistry.
