Both mutants were found to be largely helical as per design, and based on thermal denaturation experiments, were substantially more stable than the MB-1 parent molecule.
Furthermore, despite the imposed symmetric constraint upon both the primary and tertiary structure, the resulting mutant form of FGF-1 is substantially more stable.
Surprisingly, we show that heteroclitic peptides designed with optimal MHC binding motifs do not always form pMHCs that are substantially more stable than their wildtype progenitors.
The CnS− (even n = 4, 6, and 8) and CnO− (even n = 6 and 8) anions are found to be substantially more stable than their corresponding neutral species.
A small number of functional protein sequences selected from binary-patterned libraries suffice as input for the consensus design of active enzymes that are easier to produce and substantially more stable than individual members of the starting data set.
The ET data show that, under solvent conditions favoring the folded protein, collapsed non-native structures are not substantially more stable than extended conformations, and that the two populations interchange rapidly.
Among all fMRI measurements, estimates of n were the most robust and were substantially more stable within individual subjects (coefficient of variation, CV = 7.4%) than across the subject pool (CV = 36.9%).
First-order and second-order versions of the time-stepping method are derived systematically, and numerical results indicate that both methods are substantially more stable than explicit methods.
Being non-natural analogs of nucleic acids, OPS are substantially more stable towards hydrolysis by nucleases, the class of enzymes that destroy nucleic acids by breaking the bridging P-O bond of the phosphodiester moiety.
Indeed, the enzyme is substantially more stable than its shallow water orthologue, with resistance increased by over 100 MPa.
We think it likely that nucleotide occupancy at this site, in both SNF1 and AMPK, is important for the stability of the enzyme, since protein stability experiments indicate that SNF1 is substantially more stable in the presence of NADH.
