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Major order disagreements were found on BTA19, where a substantial marker gap at the proximal end of the chromosome prevented alignment of the RH and linkage maps.
However, the effects are mild in degree compared with previous studies (e.g., Hayman et al. 2003), and it does not seem that firing rate is a substantial marker of global spatial location, though it may provide a modulating influence.
Although the sequencing coverage required to generate a dense marker set seems daunting, we have demonstrated that a substantial marker density is not required, or even useful, for the purpose of QTL mapping in RI populations.
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In addition, substantial marker and linkage map resources in red clover (Trifolium pratense) [ 30] have been applied in white clover and T. subterraneum for comparative mapping [ 19, 22, 31].
All of these previous reports note significant distortion of segregation ratios for a substantial proportion of markers, rendering mapping difficult in some cases (Sauvage et al. 2010).
These data have been consolidated in two recent large meta-analyses also showing a substantial safety on markers of mineral bone disease (MBD) of this therapy [ 70, 71].
As field pea SNP discovery was based on transcriptome sequencing from multiple genotypes [ 8], it is not surprising that a substantial minority of markers (c. 35%) failed to detect polymorphism in the mapping family.
The discovery of novel plasma markers has represented a substantial challenge, particularly for markers that are applicable to early stage disease.
We present here the radiation hybrid map of chicken chromosome 2, built by using markers chosen from the GGA2 genetic map and a substantial number of additional markers developed from chicken EST data.
The chimpanzee X1 and the macaque A5 marker had a substantial number of ambiguous sites, suggesting that the two particular markers may potentially include sequencing and/or assembly errors.
A substantial number of these markers (149) were also transferable to other related species like Ficus, Fig and Jackfruit.
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