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The phrase "a substantial difference between the two" is correct and usable in written English.
It can be used when comparing two items, concepts, or situations to highlight a significant distinction.
Example: "There is a substantial difference between the two approaches to solving the problem, which could affect the outcome."
Alternatives: "a significant difference between the two" or "a marked difference between the two".
Exact(14)
There's a substantial difference between the two".
And, bingo: There was a substantial difference between the two groups' average blood concentrations of acetyl-L-carnitine.
If there is a substantial difference between the two an aid award adjustment (either increase or decrease ) may be made.
The results indicate a substantial difference between the two latent classes.
Furthermore, there is a substantial difference between the two technologies: hydrogen is an energy carrier, while fuel cells are energy converters.
As the figure shows, there is a substantial difference between the two corpora in terms of the percentage of mental and relational processes.
Similar(46)
There is a substantial difference between the exit wave intensities.
There is a substantial difference between the a.s.s
This suggests a somewhat substantial difference between the two symptoms represented by clusters 1 and 3.
In general, there were not any substantial differences between the two groups.
The substantial difference between the three analyses is well explained by the completely different permutation approaches.
More suggestions(15)
a tremendous difference between the two
a huge difference between the two
a great difference between the two
a fundamental difference between the two
a notable difference between the two
a massive difference between the two
a considerable difference between the two
a substantive difference between the two
a substantial gap between the two
a substantial partnership between the two
a slight difference between the two
a philosophical difference between the two
a second difference between the two
a sharp difference between the two
a consistent difference between the two
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com