The phrase "a substantial defect in" is correct and usable in written English.
It can be used when discussing significant flaws or issues in a product, system, or process.
Example: "The inspection revealed a substantial defect in the manufacturing process that could lead to safety concerns."
Alternatives: "a major flaw in" or "a significant issue with".
The spt16-m allele contains two amino acids substitution (K692AR693A) in its tandem pleckstrin homology (PH) domain and displays a subtle defect in transcription initiation but a substantial defect in DNA replication-coupled nucleosome assembly (Yang et al., 2016).
On the other hand, Notch1 receptors lacking the S1 loop exhibit a substantial defect in transport to the cell surface, with steady state cell-surface levels reduced by 5 10-fold, even though there are comparable levels of overall expression.
Given our failure to find a substantial defect in ErbB2 transcription or protein degradation rates, we suspected this higher molecular weight form may represent ubiquitinated ErbB2 that accumulates upon POH1 knockdown and may be less easily detectable by western blotting due to its heterologous molecular weight.
We looked at ribosome biogenesis, and were inspired when we noticed that there is a substantial defect in rRNA transcription.
The C. neoformans met3 mutant, which lacks ATP sulphurylase activity, had a substantial defect in melanin formation, significantly reducedgrowth rate, and greatly increased thermotolerance.
Key amongst these proteins is CtIP that, together with the MRE11-RAD50-NBS1 complex, plays a major role in initiating resection, with CtIP impairment causing a substantial defect in RPA-coated ssDNA accumulation after CPT treatment (15).
We next monitored the accumulation of RAD51 foci following irradiation and found a more substantial defect in INO80-depleted cells (Fig 2B).
The small size of dcko limb buds was not due to any substantial defects in expression of Fgf8 in the apical ectodermal ridge or Sonic hedgehog (Shh) in the zone of polarizing activity (Fig. 7IVA H).
We also monitored possible effects of A2ML MO injection on blood vessel formation in the liver by inspecting fliGFP transgenic animals, and found no substantial defect in blood vessel formation (Fig. S2A B).
XynA mutants were tested for growth on xylan and xylose with no substantial defect in growth, in agreement with previous results [ 34].
Vav1-deficient mice exhibit substantial defects in T cell development, marked by a block at the DN3 stage and dramatically reduced activities of ERKs and PLC- γ1[17], [18], critical downstream mediators of Vav signaling in response to TCR stimulation.
