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Representative examples of isolates possessing a PCR-RFLP genotype distinct from those that we previously identified [13], [22], [23] were used in a subsequent sequence comparison.
A subsequent sequence optimization provided a peptide with improved affinity.[ 394] Class B peptidomimetics were also used to disrupt PPIs between proteins of the BCL-2 family.
A Paramecium tetraurelia pilot genome project, the subsequent sequencing of a Megabase chromosome as well as the Paramecium genome project aimed at gaining insight into the genome of Paramecium.
This was confirmed to be a homozygous mutation by subsequent sequencing of the abnormal region.
By PCR amplification and subsequent sequencing, a BRAF V600E mutation was detected in the patient sample.
A pooled RNA sample was provided to GATC Biotech (Konstanz, Germany) for the construction of a cDNA library and subsequent sequencing.
Following RNA isolation and subsequent sequencing, a differential expression analysis was performed to identify transcriptional changes resulting from treatment with the small molecule.
Twenty-six sampositiveitive for hemoplasma by SYBR® Green qPCR underwent cPCR for amplification of a longer fragment and subsequent sequencing for comparison to 16S rRNA gene sequences available in GenBank®.
In the breast tumour sample set, sample B14 was interesting as it was scored as a query by HRM and subsequent sequencing showed the presence of a 531_533delC mutation that was present at a low percentage.
An alternative explanation could be that the duplication took place in a metazoan ancestor, but that sequences of MHCIIb are misplaced in the tree due to a higher subsequent sequence divergence.
Subsequent sequencing of a Ca-transport gene, SERCA, revealed mutations in the protein's cytosolic domain.
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