Behavioral responses were videotaped for subsequent quantification of a "listening" index [ 70].
Single-base extension of the hybridized probes incorporates a labeled ddNTP, which allows subsequent quantification of methylated and unmethylated alleles (http://www.illumina.com/technology/infinium_methylation_assay.ilmn).ilmn
Therefore, the deposition and subsequent quantification of A β load in the brain of affected individuals are imperative as detection of amyloid deposition may be the first step(s) towards diagnosis and subsequent optimization of treatment strategies for the ensuing neuropathology.
In addition, we speculated that the complex composition of the spinal segment tissue, which contains different neuronal and glial cells and different cells in the supporting connective tissue, may be the principal obstacle for a correct tissue dissection and subsequent quantification of miRNA expression.
The DNA tail was engineered to include a single ScrFI restriction site, which enabled subsequent quantification of restricted DNA tails using real-time PCR.
Enzymatic digestion using GluC and trypsin generated chemically identical heavy and light peptide pairs, which were resolved by nano-LC-MS/MS, with subsequent quantification of acetylation stoichiometry in a site-specific manner.
AC activity was tested using 30 min incubation of 0.02 μg sAC in 20 mM Tris/HCl pH 7.8, 5 mM ATP, 5 mM CaCl2 and 10 mM MgCl2 at 37°C, and subsequent quantification of cAMP formed by using an immunoassay kit (Assay Designs) as recommended by the manufacturer.
Whenever a forum post was found that was relevant to the coding framing, then the post was tagged given the appropriate framing code, and an entry was made in a separate document against the contributor's forum alias, allowing for subsequent quantification of results.
Serial dilution of plasma revealed that a difference of 20%% can be detected by immunoblot and the subsequent quantification of the signals with OptiQuant software (not shown).
Subsequent quantification of the number of motor neurons revealed a remarkable reduction in motor neuron survival in SOD1 G93A sections compared with Aptx−/− or wild-type controls (230 ± 30 SEM versus 450 ± 24 SEM; Fig. 4B).
