Accordingly, the radioactivity of all subsequent fractions was added to the radioactivity retained in the tissue.
Positioning errors ≥5 mm were considered unacceptable and were required to have a correction applied before the subsequent fraction being delivered.
The investigated patients are expected to benefit from this study, since in case of detected deviations between planned and actual treatment delivery a proper intervention (e.g., correction) could be performed in a subsequent irradiation fraction.
The residue from each fractionation step was used to obtain the subsequent fraction.
We exploit the fact that the exact date of treatment is known: in a month in which a treatment is obtained, one can distinguish the fraction of the month before the treatment, and the subsequent fraction of the month, starting on the day of the treatment.
The concentrated residue was extracted sequentially with petroleum ether, chloroform, ethyl acetate, and n-butanol, the residue from each extraction step being used to obtain the subsequent fraction.
Each subsequent fraction begins its set of integers with the highest one used in the previous fraction.
The subsequent fractions were eluted using a linear gradient of NaCl (from 0 to 2 M) (19 fractions of 1 ml each).
To investigate whether gorgonian extracts contained possible substrates for Cyphoma GSTs, we used a bioassay-guided fractionation approach, screening gorgonian crude organic extracts and subsequent fractions of different polarities for their ability to inhibit the 1-chloro-2,4-dinitrobenzene (CDNB -conjugating aCDNB -conjugating
Because the diverse array and types of chemical components in propolis vary in size and polarity, the solvents used to extract the propolis play a key role in the bioactivities, including anti-cancer activities, that are obtained in the crude extracts or subsequent fractions [ 16], due to the differential fractionation of components between different extracting solvents.
So the 2006 legislation capped the deduction at the value prevailing when the donor gave away the first fraction or subsequent fractions, whichever was lowest.
