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This method combines a strong fixation to preserve the chromosomal structures, acetic acid spreading to remove the cytoplasm and treatment in microwave (850 W) to increase the access of the antibody to the protein.
At the same time, these mutations undergo a strong fixation bias during their propagation into populations in such a way that fixed substitutions tend to preserve MRI regions.
However, as the ChIP procedure requires a strong fixation, which permanently crosslinks DNA and TR, it can only provide a snapshot of the highly dynamic events taking place in vivo.
On the one hand, a strong fixation to the loved object must have been present; on the other hand, in contradiction to this, the object-[investment] must have had little power of resistance … This contradiction seems to imply that the object-choice had been effected on a narcissistic basis, so that the object-[investment], when obstacles [came] in its way, [could] regress to narcissism.
Consistent with our earlier study, synonymous site sequence evolution is most extreme at Notch, with a strong fixation bias toward unpreferred codons on the D. melanogaster lineage and a contrastingly strong fixation bias toward preferred codons on the D. sechellia/ D. simulans lineage (Bauer DuMont et al. 2004; Singh et al. 2007).
Following our previous observation of a strong fixation bias in favor of derived C/G alleles at CG-related sites[ 23], we re-examined the mutation and fixation pattern of CG dinucleotides on a genome-wide scale, while taking the sequence context into consideration.
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Lastly, an interference-fit test model was used to demonstrate that mechanically-driven SMPs could immediately create and maintain a stronger fixation force compared to as-machined samples and thermally-driven SMP samples.
The stacked suture created a stronger fixation than the parallel suture.
The AFRIF fixation technique provided a stronger fixation than TPF, LPF, and HPF.
It is imperative that a predictable and strong fixation to the pectoralis major fascia be obtained because the muscle fibers alone are prone to rupture.
Converging screws may also be used on the rare occasions when the femoral neck is too narrow to accommodate two parallel screws and for subtrochanteric fractures which are known to benefit from strong fixation with an intramedullary device [9].
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