Cotransfection of this construct with Pdu-bmal and Pdu-clock into S2 cells led to a strong activation of luciferase activity.
Moreover, the loss of functional changes in the two major tumor suppressor proteins pRB and TP53 activities induced a strong activation of ribosome biogenesis in cancer cell [24].
At the same time, the level of phospho-active JNK was assessed by confocal imaging, a strong activation of the kinase being evident within 1 h of treatment.
Although a strong activation of PKA leads to IPC cell death, the basal activity seemed necessary for growth.
Similarly to the perfused heart experiments, we saw a strong activation of ASK1 during ischaemia which correlated with increased p38 activity (Fig. 5B).
Thus, the association of Abp1 with the Arp2/3 complex-activator N-WASP does not compete with the binding of Cdc42 but both activators act in concert and in combination lead to a strong activation of N-WASP.
Using a reporter plasmid for NF-κB activation, it was confirmed that dCGN induced a strong activation of NF-κB, as reflected by an increase in luciferase activity (Fig. 7A).
On the contrary, two independent FGF-iPSC lines displayed a strong activation of Smad 2/3 concomitant with undetectable levels of Smad 1/5/8 active forms (Fig. 5C).
The expression of lethal doses of MEKK1 resulted in a strong activation of all three major MAP kinase families JNK, ERK, and p38.
Cerebral ischemia induces the death of ischemic neurons and especially the release of necrotic debris that triggers the inflammatory reaction resulting in a strong activation of the resident glial cells and leucocyte infiltration [1].
Foremost, the contrast revealed a strong activation of the amygdala.
