If we impose a more stringent strategy (i.e. P < 0.05 in both conditions except for tPAV genes which are expressed in only one condition), only 351 genes are shortlisted (data not shown).
As the aim of this study was to understand the molecular mechanisms underlying adaptation to long-term exposure to heat stress in Pecten maximus, a very stringent strategy was adopted to identify the consistently differentially expressed transcripts.
It is important to note that this discovery was made possible by the stringent strategy we employ which avoids assigning an organism to a lower-level taxonomic group if the evidence does not support this assignment, a feature not available in other taxonomic profiling tools.
A stringent selection strategy resulted in the identification of 60 unique HIV-1 peptide phage, which were subjected to sequence analysis and mapped onto the ITM1 envelope sequences.
To identify novel miRNA precursor structures, we applied a stringent filtering strategy [38] to the RNAz output, selecting only RNAz predictions with i) a stem-loop structure exceeding 20 nt, and ii) high predicted thermodynamic stability, as indicated by a z-score of less than −3.5.
For selection of highly reliable expression data and validation of the miRDeep prediction, we decided to apply a stringent selection strategy.
To accurately monitor HbF and HbA co-expression by flow cytometry, we firstly generated a stringent gating strategy developed using appropriate cellular controls (Fig 5).
28 The building of the golf estate, however, would lead to the establishment of a gated community along the border with a stringent security strategy including an electric fence system and regular patrols in place (Strategic Environment Focus 2006b).
When employing a stringent sorting strategy that formally excludes RBCs, the DNAm relationships between cord blood T cells, monocytes, and nRBCs were more consistent with previous hematopoietic lineage studies [ 2, 26– 29].
