Five years ago, the state first intervened in Roosevelt schools, and in January 1996, the Regents took a more stringent step when it ordered the school board removed.
In a first stringent step, we removed 18,274 A. thaliana and 28,482 O. sativa proteins tagged with the same PFAM motifs.
Strict monotonicity, in terms of arbitrary starting values for the multistep schemes, is only valid for a small class of methods, under very stringent step size restrictions.
A stringent washing step (RIPA buffer) was included in our binding assay to reduce non-specific binding of actin to agarose beads (Fig. 1C).
Briefly, the pool of SP candidates was generated by a data-dependent nano-LC-LTQ/Orbitrap analysis and a stringent filtering step.
Here, a BLAST homology search of the A. digitifera predicted proteome against the UniProtKB/Swiss-Prot Tox-Prot dataset, followed by a stringent five step process to exclude proteins with possible non-toxic physiological functions [ 41– 43], uncovered 55 potential toxins with homology to higher animal toxins (Table 1).
The fourth and most stringent step is testing for strict factorial invariance.
After overnight hybridisation (2 × SSC, 50% formamide, 10% dextran sulphate, 55°C) with an S-labelled RNA probe (1.16 × 10 c.p.m. μl−1), sections were washed (the most stringent step being 0.1 × SSC, 15 min at 60°C) and treated with RNAse A (20 μg ml−1, 37°C, 30 min).
The first and most stringent step imposed the requirement that the blast match_length must be equal to the smallest of the two sequences (query or subject) and the number of mismatches = 0, number of gaps = 0, and the percent identity ≥ 99%.
The PICC site requires a stringent, multi-step cleaning and redressing process, and I took careful notes from the visiting nurse when she came to change the dressing.
The diagnostics of OCD were based on a stringent three-step procedure.
