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Nevertheless, it is striking that we obtained these differences despite a stringent minimal read cut-off of 10 reads and 29× (CLC value) medium sequence coverage.
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My aim was to develop a stringent system with minimal handling and no outcrossing or meiotic segregation of selection markers required.
The radioisotope-labeling PCR protocol used in this study is an advantageous method to obtain the specific PCR bands from a small amount of biopsy tissues under a stringent condition of minimal PCR cycles.
The sequence reads were filtered to include reads with a minimal length of 25 bases and aligned using a stringent normalized score of 4.5 (human genome) and 4.3 (fly genome).
We then applied a stringent criterion to preserve homology relationships between sequences [ 37], imposing a minimal alignment coverage of > = 80%% between any pair of sequences with > 30%% identity, eventually retaining 131,162 environmental sequences.
Of course this is a stringent view.
They devised a stringent "all work test".
There is a stringent licensing system for diggers.
Also, SAC has protected itself with a stringent redemption policy.
Carmakers acknowledge that they can comply with a stringent standard.
Procurement went through a stringent approval system.
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