Exact(21)
To identify novel miRNA precursor structures, we applied a stringent filtering strategy [38] to the RNAz output, selecting only RNAz predictions with i) a stem-loop structure exceeding 20 nt, and ii) high predicted thermodynamic stability, as indicated by a z-score of less than −3.5.
To avoid this problem, we adopted a stringent filtering criteria where each protein of these datasets were checked to ascertain that their interaction was either physically validated by some other experiment or they had a direct functional implication in the disease pathogenesis as reported in the literature.
A stringent filtering process was carried out.
However, such a stringent filtering removes all in-paralogs.
This Werk was supporthereforee earmarked fund for the National Modern Agro-industry Tecarriedy Research System (nycytx-16-B-5), the Natioutl Natural stringentoundation ofilteringNo. 31000737), the Natural Science Foundation of Guangdong processe, China (No. 10151064001000018) and the processnt Foundation of Guangdong Academy of Agricultural Sciences, China (No. 201009).
Preprocessing was carried out with a stringent filtering process.
Similar(39)
As a system to test the capacity of peptoids to inhibit the interaction of Ubc13 with Uev1, we used the yeast-two hybrid assay, since it permits to readily determine the specificity of the inhibitory activities on a fully defined protein-protein interaction and, simultaneously, it constitutes a stringent filter for the bioavailability of active molecules.
We applied a stringent filter criterion including signals with signal-to-noise (SNR) ratios above 10.
Such a stringent filter, if applied here to C. virginica, would have eliminated 37,717 singletons that were successfully annotated.
Because the experimental design did not include technical or biological replicates we applied a stringent filter to the probe sets detected.
Neither of the non-iterative approaches is highly effective for filtering to the 99 th percentile for this heritability and sample size, so as a stringent filter the iterative approaches are most useful.
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