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In addition, as it can be seen in Figure 2, SMARCA4 correlates with SECISBP2 through GPX1, thus suggesting a stringent correlation between SMARCA4, SP1, SECISBP2, and many other mRNAs.
Unfortunately, from the many mechanisms, no single one shows a stringent correlation with the inhibition of bone resorption in vivo when different bisphosphonates of various potencies are investigated.
Since the above result establishes a stringent correlation between EGFR ubiquitination and EGFR-NCE, we exploited the add-back mutants to investigate whether they are also mechanistically linked.
The choice of GAPDH as a reference gene was based on GAPDH having a stringent correlation in the lung cells and the least variation of the seven common reference genes [ 21].
Despite this general picture, the exact topography of the dopaminergic and serotonergic nerve fibers, their varicosities, and/or their terminals with respect to the various structures and cell types engaged in saliva production and modification is still insufficient for a stringent correlation of innervation and gland physiology.
We chose such a stringent correlation threshold considering the relatively small sample size in the analysis so that gene pairs in the coexpression network are statistically significant (P = 0.0005 using Fisher's Z transformation), meaning the probability of randomly obtaining a correlation coefficient of ≥ 0.90 in this seed transcriptome dataset is small.
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To ensure only tightly correlated probes were clustered, we employed a stringent cutoff (correlation distance cutoff 0.2).
At last, we calculated the correlation coefficients of each pair of module eigengenes that stand for the first principal component of the module and merged highly similar modules by a stringent threshold (correlation > 0.9).
More stringent correlation thresholds reduce the number of genes correlated to at least one other gene at or above that threshold.
For none of these many distinct protein factors a rate-limiting role has been demonstrated yet, and no stringent correlation could be shown in human tumour cell lines between functional removal of cisplatin-DNA adducts and the expression profiles of particular repair proteins, neither at the mRNA nor at the protein level (Wang et al, 2011b).
This stringent correlation threshold was chosen to eliminate potential spurious correlations in a coexpression network.
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Justyna Jupowicz-Kozak
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