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Here, we describe a straightforward sample pretreatment step for the colorimetric cobaltthiocyanate determination of polysorbate, which circumvents the assay's shortcomings due to interference of protein and does not require complex instrumentation.
A straightforward sample preparation step was used for the assay, as described in the kit's user manual.
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In summary, the evaluation of the sensitivity, accuracy and genotyping performance provides strong support for the application of MPS technology in forensic SNP analysis, and the assay offers a straightforward sample-to-genotype workflow that could be beneficial in forensic casework with respect to both individual identification and complex kinship issues.
The present survey had a straightforward sampling plan (a nationally representative sample in terms of age and gender), which served to control costs; a more-complex quota plan (e.g., introducing a requirement for a minimum number of respondents that do not hold a driving license) would have had corresponding impacts on resource requirements for this study.
Urine samples are collected less often in large cohort studies, but they offer some advantages, notably higher polyphenol concentrations when compared with plasma and a more straightforward sample processing before analysis.
A simple and straightforward sample preparation method was developed consisting out of a deproteinisation step with acetonitrile, followed by evaporation of the supernatant and reconstitution in water.
The complete tip is used during a simple and straightforward sample preparation.
Since many clinicians were involved in the present study, it was important to establish a simple, straightforward sampling protocol in order to achieve good quality and reliable data.
On the other hand MALDI-TOF based methods to identify SNP-mutations also have an inherent capacity to combine straightforward sample preparation with high sensitivity and high-throughput and are well documented, especially in clinical applications [ 36, 56, 57].
The semi-circular bend (SCB) test has been frequently utilised due to its straightforward sample preparation, ease of test execution and applicability to studies of asphalt crack resistance.
However, the ability of Nanoannotator to resolve individual particles when they are touching each other or even overlapping to some extent enables faster and straightforward sample preparation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com