In eukaryotes, each cell division cycle is therefore executed as a tightly regulated, stepwise program that relies on intact chromosomes.
Model-based optimization reveals the beneficial effect of the use of a stepwise temperature program, as confirmed experimentally via a lowering of the dispersity from a conversion of 0.3 on due to a suppression of thermal initiation and chain transfer to dimer.
We conclude that a stepwise PD program is essential for incident PD patients.
A stepwise screening program in 190 general practices in Denmark was used to identify 1,533 people with type 2 diabetes.
This study was supported in part by a Grant-in-Aid for Cancer Research and a Grant-in-Aid for Scientific Research (A) from the Ministry of Education, Science and Culture of Japan to S.T., and the second step of Visionary Research Grant (a stepwise encouraging program) by Takeda Science Foundation to S.K.
Thailand has also made progressive changes, introducing into the accreditation process a stepwise recognition program in 2004 and patient safety goals in 2006 (See Additional file 1, Leadership and Strategy for Improvement (Thailand) and Additional file 2, Malaysian Health Sector for more information).
Seventy cycles of stepwise program (94°C for 1 min, 50°C for 2 min and 72°C for 3 min) were carried out.
Isoelectric focusing (IEF) was run following a stepwise incremental voltage program on an IPGphor Isoelectric Focusing System (GE Healthcare Life Sciences, Taipei, Taiwan).
IEF strips (pH 3 10, IPGphor, Amersham Biosciences, Uppsala, Sweden) were developed through a stepwise incremental voltage program: 30 V for 16 hr, 500 V for 1 hr, 1000 V for 1 hr, and 8000 V for 4 hr, with a total power of 34 kV hr.
